Custom Peptide Synthesis: When You Need It and How to Order

Off-the-shelf catalog products cover ~80% of research needs. For the rest, custom synthesis opens up possibilities. Here’s a practical guide.

When You Need Custom Synthesis

  • Novel sequences — your research targets a unique peptide
  • Specific modifications — acetylation, amidation, fluorescent labels
  • Unusual purity specs — need ≥99.5% for sensitive assays
  • Special scales — need 100mg+ or just 500µg
  • Stable isotope labeling — for NMR or mass spec studies

Information to Provide

To get an accurate quote, prepare:

1. Sequence

  • One-letter amino acid code
  • N-terminal and C-terminal modifications (if any)
  • Disulfide bonds (if applicable)

2. Specifications

  • Purity: ≥85% (crude), ≥95% (standard), ≥98% (premium)
  • Quantity: mg or g
  • Form: lyophilized powder or solution

3. Modifications

  • Acetylation (N-terminal)
  • Amidation (C-terminal)
  • Fluorescent labels (FITC, FAM, TAMRA)
  • Phosphorylation, glycosylation
  • D-amino acids
  • Stable isotopes (15N, 13C)

4. QC Requirements

  • HPLC only
  • HPLC + MS
  • HPLC + MS + NMR
  • Full characterization package

Typical Lead Times

Quantity Standard Rush
<10 mg 2-3 weeks 1 week
10-100 mg 3-4 weeks 2 weeks
100 mg - 1 g 4-6 weeks 3 weeks
>1 g 6-8 weeks 4 weeks

Cost Drivers

Major cost factors:

  1. Sequence length — longer = more expensive
  2. Amino acid composition — some residues cost more
  3. Modifications — each adds cost
  4. Purity target — higher purity = more purification rounds
  5. Quantity — economies of scale

Success Tips

✅ Provide as much sequence context as possible — helps us anticipate synthesis challenges ✅ Request smaller scale first — verify the product before committing to large scale ✅ Order buffer quantities — running out mid-experiment is painful ✅ Discuss QC upfront — extra characterization costs less when planned in

How to Order from Jike Bio

  1. Email sales@jikebio.com with your specs
  2. We respond within 24 hours with feasibility + quote
  3. You approve → 50% deposit → synthesis starts
  4. We send QC report for your approval
  5. Balance payment → shipment

We welcome challenging sequences — that’s where our expertise shines.